Anti-LAMP1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P11279
Gene Id	3916
Gene Symbols	LAMP1
Protein Name / Synonyms	LAMP1, CD107a, LAMPA, LGP120, lysosomal associated membrane protein 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

38kDa/44kDa

Observed MW

120kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 80-280 of human LAMP1 (NP_005552.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using MCL1 antibody (144-02582) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - LAMP1 Polyclonal Antibody (144-02582)

Immunohistochemistry of paraffin-embedded mouse kidney using LAMP1 antibody (144-02582) at dilution of 1:100 (40x lens).
Immunohistochemistry - LAMP1 Polyclonal Antibody (144-02582)

Immunofluorescence analysis of HeLa cells using LAMP1 antibody (144-02582) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - LAMP1 Polyclonal Antibody (144-02582)

Immunofluorescence analysis of NIH/3T3 cells using LAMP1 antibody (144-02582) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - LAMP1 Polyclonal Antibody (144-02582)

Expiration:

12 months from the date of shipment when stored properly.

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