Anti-LEF1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9UJU2
Gene Id	51176
Gene Symbols	LEF1
Protein Name / Synonyms	LEF1, LEF-1, TCF10, TCF1ALPHA, TCF7L3, lymphoid enhancer-binding factor 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

23kDa/31kDa/34kDa/35kDa/41kDa/42kDa/44kDa

Observed MW

40kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 100-399 of human LEF1 (NP_057353.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using LEF1 antibody (144-60041) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - LEF1 Polyclonal Antibody (144-60041)

Expiration:

12 months from the date of shipment when stored properly.

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