Anti-LEP Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P41159
Gene Id	3952
Gene Symbols	LEP
Protein Name / Synonyms	LEP, LEPD, OB, OBS, leptin
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Angiogenesis, Cancer, Inflammation, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development.

Dilutions

WB 1:200 - 1:500

Format

Calculated MW

18kDa

Observed MW

17kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 22-167 of human LEP (NP_000221.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of mouse pancreas, using LEP antibody (144-01300) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - LEP antibody (144-01300)

Expiration:

12 months from the date of shipment when stored properly.

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