Anti-LEPR Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Rat
Host Species	Rabbit
Accession Number	P48357
Gene Id	3953
Gene Symbols	LEPR
Protein Name / Synonyms	LEPR, CD295, LEP-R, LEPRD, OB-R, OBR, leptin receptor
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Angiogenesis, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).

Dilutions

WB 1:500 - 1:1000
IHC 1:50 - 1:200

Format

Calculated MW

96kDa/102kDa/103kDa/109kDa/132kDa

Observed MW

113kDa

Immunogen

A synthetic peptide of human LEPR

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using LEPR antibody (144-02999) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - LEPR Polyclonal Antibody (144-02999)

Expiration:

12 months from the date of shipment when stored properly.

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