3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-LHX4 Antibody
Catalog #: 144-08072
LHX4 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q969G2 |
| Gene Id | 89884 |
| Gene Symbols | LHX4 |
| Protein Name / Synonyms | LHX4, CPHD4, LIM homeobox 4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein is a transcription factor involved in the control of differentiation and development of the pituitary gland. Mutations in this gene cause combined pituitary hormone deficiency 4.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
43kDa
Observed MW
43kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 121-390 of human LHX4 (NP_203129.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using LHX4 Antibody (144-08072) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using LHX4 antibody (144-08072) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human oophoroma using LHX4 antibody (144-08072) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using LHX4 antibody (144-08072) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using LHX4 antibody (144-08072) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using LHX4 antibody (144-08072) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse cancer using LHX4 antibody (144-08072) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.