Anti-LIG1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P18858
Gene Id	3978
Gene Symbols	LIG1
Protein Name / Synonyms	LIG1, DNA ligase 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

88kDa/98kDa/101kDa

Observed MW

140kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 565-790 of human LIG1 (NP_000225.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using LIG1 antibody (144-01858) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - LIG1 antibody (144-01858)

Immunofluorescence analysis of MCF-7 cells using LIG1 antibody (144-01858). Blue: DAPI for nuclear staining.
Immunofluorescence - LIG1 antibody (144-01858)

Immunofluorescence analysis of A549 cells using LIG1 antibody (144-01858).
Immunofluorescence - LIG1 antibody (144-01858)

Expiration:

12 months from the date of shipment when stored properly.

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