Anti-LIM kinase 2 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	3985
Protein Name / Synonyms	LIM kinase 2
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Research Area	Phosphorylation
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

LIMK2 antibody, LIM domain kinase 2 antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

72 kDa

Immunogen

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 294 and 655 of LIM kinase 2

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using LIM kinase 2(107-10712) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.

Immunohistochemical analysis of paraffin-embedded CA922 xenograft , using LIM kinase 2(107-10712) antibody at 1:500 dilution.

Sample (30µg whole cell lysate)
A:293T
7.5% SDS PAGE
107-10712 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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