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Anti-LIMS1 Antibody
Catalog #: 144-06384
LIMS1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P48059 |
| Gene Id | 3987 |
| Gene Symbols | LIMS1 |
| Protein Name / Synonyms | LIMS1, PINCH, PINCH-1, PINCH1, LIM zinc finger domain containing 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
37kDa/38kDa/41kDa/44kDa
Observed MW
40kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human LIMS1 (NP_001180417.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of HepG2 cells, using LIMS1 antibody (144-06384) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunofluorescence analysis of MCF7 cells using LIMS1 antibody (144-06384). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.