Anti-LIN28B (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q6ZN17
Gene Id	389421
Gene Symbols	LIN28B
Protein Name / Synonyms	LIN28B (N-term)
Clonality	Polyclonal
Preparation	This LIN28B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LIN28B.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	IF, FC, Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Lin-28 homolog B (LIN28B) is overexpressed in hepatocellular carcinoma. The heterochronic gene lin-28 is a key regulator of developmental timing in the nematode Caenorhabditis elegans. Similar with lin-28 proteins, LIN28B conserves a cold shock domain and a pair of CCHC zinc finger domains. Phylogenetic analysis suggests that they might arise as a result of duplication from an ancestral gene. Overexpression of LIN28B was noted in most HCC cell lines and clinical samples. A short LIN28B isoform was also identified in non-tumor liver tissue and fetal liver. Although predominantly localized in the cytoplasm, LIN28B protein shows cell cycle-dependent nuclear translocation in Huh7 cells. Induced expression of exogenous LIN28B in a tet-off cell line promoted cancer cell proliferation.

Specificity

Other Names

LIN28B; CSDD2; Protein lin-28 homolog B

NCBI Accession #

NP_001004317.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

27084

Recommended Dilutions

IF: 1:100; WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	Fluorescent confocal image of SY5Y cells stained with LIN28B (N-term) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-12692 LIN28B (N-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). Note the highly specific localization of the LIN28B immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000187772).
	Western blot analysis of LIN28B Antibody (N-term) (Cat# 102-12692) in HL60 cell line lysates (35ug/lane). LIN28B (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human Testis tissue reacted with LIN28B Antibody (N-term)(Cat# 102-12692), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of HL-60 cells using LIN28B Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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