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Anti-LMNA Antibody
Catalog #: 144-00249
LMNA Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P02545 |
| Gene Id | 4000 |
| Gene Symbols | LMNA |
| Protein Name / Synonyms | LMNA, CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1, lamin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
62-74kDa
Observed MW
69kDa, 78kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 403-572 of human Lamin C (NP_005563.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using Lamin A/C antibody (144-00249) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37? for 20 hours or treated by Paclitaxel (100 nM/ml) at 37? for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37? for 20 hours or treated by Paclitaxel (100 nM) at 37? for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat brain using Lamin A/C antibody (144-00249) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using Lamin A/C antibody (144-00249) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using Lamin A/C antibody (144-00249) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using Lamin A/C antibody (144-00249) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded Human colon using Lamin A/C antibody (144-00249) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human esophageal using Lamin A/C antibody (144-00249) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using Lamin A/C antibody (144-00249) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human gastric using Lamin A/C antibody (144-00249) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human gastric cancer using Lamin A/C antibody (144-00249) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human kidney using Lamin A/C antibody (144-00249) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human kidney cancer using Lamin A/C antibody (144-00249) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human liver injury using Lamin A/C antibody (144-00249) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using Lamin A/C antibody (144-00249) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat heart using Lamin A/C antibody (144-00249) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using Lamin A/C antibody (144-00249). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.