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Anti-LMNB1 Antibody

Catalog #: 144-01910

LMNB1 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P20700
Gene Id
4001
Gene Symbols
LMNB1
Protein Name / Synonyms
LMNB1, ADLD, LMN, LMN2, LMNB, lamin-B1
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.09% Sodium azide, 50% glycerol, pH 7.3
Recommended ApplicationsWestern Blotting, IHC, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW
66kDa
Observed MW
70kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 397-586 of human LMNB1 (NP_005564.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using LMNB1 antibody (144-01910) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - LMNB1 Polyclonal Antibody (144-01910)

Western blot analysis of extracts from normal (control) and LMNB1 knockout (KO) HeLa cells, using LMNB1 antibody (144-01910) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - LMNB1 Polyclonal Antibody (144-01910)

Immunohistochemistry of paraffin-embedded rat spinal cord using LMNB1 antibody (144-01910) at dilution of 1:100 (40x lens).
Immunohistochemistry - LMNB1 Polyclonal Antibody (144-01910)

Immunohistochemistry of paraffin-embedded rat heart using LMNB1 antibody (144-01910) at dilution of 1:100 (40x lens).
Immunohistochemistry - LMNB1 Polyclonal Antibody (144-01910)

Expiration:
12 months from the date of shipment when stored properly.