RayBiotech

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Anti-LPAR2 Antibody

Catalog #: 144-60810

LPAR2 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Accession Number
Q9HBW0
Gene Id
9170
Gene Symbols
LPAR2
Protein Name / Synonyms
LPAR2, EDG-4, EDG4, LPA-2, LPA2, lysophosphatidic acid receptor 2
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of family I of the G protein-coupled receptors, as well as the EDG family of proteins. This protein functions as a lysophosphatidic acid (LPA) receptor and contributes to Ca2+ mobilization, a critical cellular response to LPA in cells, through association with Gi and Gq proteins. An alternative splice variant has been described but its full length sequence has not been determined.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
39kDa
Observed MW
38kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human LPAR2 (NP_004711.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of HL-60 cells, using LPAR2 antibody (144-60810) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - LPAR2 antibody (144-60810)

Expiration:
12 months from the date of shipment when stored properly.