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Anti-LRP5 Antibody
Catalog #: 144-00130
LRP5 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O75197 |
| Gene Id | 4041 |
| Gene Symbols | LRP5 |
| Protein Name / Synonyms | LRP5, BMND1, EVR1, EVR4, HBM, LR3, LRP-5, LRP7, OPPG, OPS, OPTA1, VBCH2, LDL receptor related protein 5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a transmembrane low-density lipoprotein receptor that binds and internalizes ligands in the process of receptor-mediated endocytosis. This protein also acts as a co-receptor with Frizzled protein family members for transducing signals by Wnt proteins and was originally cloned on the basis of its association with type 1 diabetes mellitus in humans. This protein plays a key role in skeletal homeostasis and many bone density related diseases are caused by mutations in this gene. Mutations in this gene also cause familial exudative vitreoretinopathy. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
179kDa
Observed MW
150kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1000-1250 of human LRP5 (NP_002326.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using LRP5 antibody (144-00130) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver using LRP5 antibody (144-00130) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using LRP5 antibody (144-00130) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using LRP5 antibody (144-00130) at dilution of 1:200 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.