LRPPRC Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P42704 |
| Gene Id | 10128 |
| Gene Symbols | LRPPRC |
| Protein Name / Synonyms | LRPPRC, CLONE-23970, GP130, LRP130, LSFC, leucine rich pentatricopeptide repeat containing |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a leucine-rich protein that has multiple pentatricopeptide repeats (PPR). The precise role of this protein is unknown but studies suggest it may play a role in cytoskeletal organization, vesicular transport, or in transcriptional regulation of both nuclear and mitochondrial genes. The protein localizes primarily to mitochondria and is predicted to have an N-terminal mitochondrial targeting sequence. Mutations in this gene are associated with the French-Canadian type of Leigh syndrome.
Dilutions
WB 1:1000 - 1:2000
IHC 1:100 - 1:200
IF 1:100 - 1:200
IHC 1:100 - 1:200
IF 1:100 - 1:200
Format
Calculated MW
157kDa
Observed MW
140kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1041-1394 of human LRPPRC (NP_573566.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using LRPPRC antibody (144-03365) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Immunohistochemistry of paraffin-embedded human lung cancer using LRPPRC antibody (144-03365) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using LRPPRC antibody (144-03365) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using LRPPRC antibody (144-03365) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using LRPPRC antibody (144-03365) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using LRPPRC antibody (144-03365) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.