Anti-LSD1 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O60341
Gene Id	23028
Gene Symbols	KDM1A
Protein Name / Synonyms	LSD1 (Center)
Clonality	Polyclonal
Preparation	This LSD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 457-490 amino acids from the Central region of human LSD1.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.

Specificity

Predicted Reactivity

Mouse

Other Names

KDM1A; AOF2; KDM1; KIAA0601; LSD1; Lysine-specific histone demethylase 1A; BRAF35-HDAC complex protein BHC110; Flavin-containing amine oxidase domain-containing protein 2

NCBI Accession #

NP_001009999.1;NP_055828.2

Other Accession #

Q6ZQ88

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

92903

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of AOF2 (arrow) using LSD1 Antibody (Center) (Cat# 102-11439). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOF2 gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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