RayBiotech

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ISO 13485:2016 cGMP

Anti-LSM2 Antibody

Catalog #: 144-07988

LSM2 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Accession Number
Q9Y333
Gene Id
57819
Gene Symbols
LSM2
Protein Name / Synonyms
LSM2, C6orf28, G7B, YBL026W, snRNP, U6 snRNA-associated Sm-like protein LSm2
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of the LSm family of RNA-binding proteins. LSm proteins form stable heteromers that bind specifically to the 3'-terminal oligo(U) tract of U6 snRNA and may play a role in pre-mRNA splicing by mediating U4/U6 snRNP formation. Pseudogenes of this gene are located on the short arm of chromosomes 6 and 19.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW
10kDa
Observed MW
13kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human LSM2 (NP_067000.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using LSM2 antibody (144-07988) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
Western blot - LSM2 antibody (144-07988)

Immunohistochemistry of paraffin-embedded human gastric cancer using LSM2 antibody (144-07988) at dilution of 1:100 (40x lens).
Immunohistochemistry - LSM2 antibody (144-07988)

Immunofluorescence analysis of U2OS cells using LSM2 antibody (144-07988) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - LSM2 antibody (144-07988)

Expiration:
12 months from the date of shipment when stored properly.