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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9Y4Z0 |
| Gene Id | 25804 |
| Gene Symbols | LSM4 |
| Protein Name / Synonyms | LSM4, GRP, YER112W, U6 snRNA-associated Sm-like protein LSm4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a member of the LSm family of RNA-binding proteins. LSm proteins form stable heteromers that bind specifically to the 3'-terminal oligo(U) tract of U6 snRNA and may play a role in pre-mRNA splicing by mediating U4/U6 snRNP formation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:200
IHC 1:50 - 1:200
IP 1:50 - 1:200
Format
Calculated MW
15kDa
Observed MW
15kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-139 of human LSM4 (NP_036453.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using LSM4 antibody (144-05891) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot analysis of extracts of various cells, using LSM4 antibody (144-05891) at 1:1000 dilution ratio through one-step method.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3min.
Immunohistochemistry of paraffin-embedded human mammary cancer using LSM4 antibody (144-05891) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using LSM4 antibody (144-05891) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using LSM4 antibody (144-05891) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.
