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Anti-LYK5 (N-term) Antibody
Catalog #: 102-27184
Rabbit Anti-LYK5 (N-term) Antibody, 400 µl
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q7RTN6|Q3UUJ4 |
| Gene Id | 92335 |
| Gene Symbols | STRADA |
| Protein Name / Synonyms | STE20-related kinase adapter protein alpha, STRAD alpha, STE20-related adapter protein, Serologically defined breast cancer antigen NY-BR-96, STRADA, LYK5 {ECO:0000312|EMBL:AAP422801}, STRAD |
| Clonality | Polyclonal |
| Clone | RB8539 |
| Isotype | Kappa, Rabbit |
| Recommended Applications | FC, Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
LYK5, also STE20-related Adaptor Protein (STRAD) are members of STE-20 like kinase family that are known to stimulate MAPK pathways by directly activating MAPKKK. LYK5 is a novel pseudokinase member of this family consisting of a STE-20 like kinase domain but lacks several residues that are required for its catalytic activity. It specifically binds LKB1 and plays a key role in regulating tumor suppressor activities of LKB1. It functions as an upstream activator of LKB1 and also directs the sub-cellular localization of LKB1 by anchoring it in the cytoplasm. STRAD-LKB1 interaction results in phosphorylation of STRAD and enhanced autophosphorylation of LKB1.
Other Information
NCBI Accession #
NP_001003786.1;NP_001003787.1;NP_001003788.1;NP_001159441.1;NP_001159442.1;NP_699166.2
Antigen Type
Synthetic Peptide
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Calculated Molecular Weight (Da)
48369
Antigen Source
Human
Images
FC (1:25)
Overlay histogram showing A549 cells stained with 102-27184(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (102-27184, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1?g/1x10
Expiration:
12 months from the date of shipment when stored properly.