3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-m1A Antibody
Catalog #: 131-0172
N1-methyladensoine (m1A) Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg, 1 mg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Clonality | Polyclonal |
| Preparation | This antibody was produced from antiserum of rabbits immunized with a recombinant protein immunogen. The IgG fraction was purified by Protein A affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution = 1:5, 000) |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in glycine solution. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Mono-methylation of adenosine in the N1 position, N1-methyladensoine (m1A), is a reversible modification that was first discovered in tRNA in 1966. Decades later, m1A was found in tRNA, rRNA, mitochondrial RNA and mRNA. It has been verified that m1A dysregulation affects multiple cellular processes, including RNA structural stability, folding, interactions with proteins, cell viability, impaired self-renewal ability, cell proliferation, and cell death. They tend to be conserved and have been implicated in many critical biological processes including development, disease, circadian rhythm and embryonic stem cell fate transition, as well as age-related disorders such as Alzheimer’s disease.
Indirect Fluorescent Antibody (IFA) Analysis
Alexa Fluor 488 goat anti-rabbit (1:200)
Hela cells
Subcellular Location: extracellular space
CY3 goat anti-rabbit (1:200)
Hela cells
Subcellular Location: extracellular space
Storage/Stability
Store at -20°C. If intended for use within a month, may keep at 4°C. Minimize freeze-thaw cycles.
Expiration
12 months from the date of shipment when stored properly.