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Anti-m7G Antibody
Catalog #: 131-0171
N7-methylguanosine (m7G) Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg, 1 mg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Clonality | Polyclonal |
| Preparation | This antibody was produced from antiserum of rabbits immunized with a recombinant protein immunogen. The IgG fraction was purified by Protein A affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution = 1:5, 000) |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in glycine solution. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
N7-methylguanosine (m7G) is the most ubiquitous mRNA cap modification and is also present in internal mRNA, microRNA (miRNA), tRNA and rRNA. m7G is positively charged and produced by the methyltransferase-like 1 (METTL1) and WD repeat domain 4 (WDR4) complex. Emerging evidence suggests that the METTL1/WDR4 complex promoted or inhibited the processes of brain development and diseases, as well as many kinds of tumor, including head and neck, lung, liver, colon, bladder cancer, and teratoma. Additionally, m7G is also crucial for tumor chemoresistance, vasculogenesis and prognosis. Therefore, m7G modification can be used as biomarkers or potential intervention targets, providing new possibilities for early diagnosis and treatment of tumors.
Indirect Fluorescent Antibody (IFA) Analysis
Alexa Fluor 488 goat anti-rabbit (1:200)
Hela cells
Subcellular Location: extracellular space
CY3 goat anti-rabbit (1:200)
Hela cells
Subcellular Location: extracellular space
Immunohistochemistry (IHC) Analysis
Storage/Stability
Store at -20°C. If intended for use within a month, may keep at 4°C. Minimize freeze-thaw cycles.
Expiration
12 months from the date of shipment when stored properly.