Anti-MAD2L2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q9UI95
Gene Id	10459
Gene Symbols	MAD2L2
Protein Name / Synonyms	MAD2L2, FANCV, MAD2B, POLZ2, REV7, mitotic arrest deficient 2 like 2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The encoded protein, which is similar to MAD2L1, is capable of interacting with ADAM9, ADAM15, REV1, and REV3 proteins.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

24kDa

Observed MW

24kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human MAD2L2 (NP_006332.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using MAD2L2 antibody (144-09861) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - MAD2L2 antibody (144-09861)

Immunohistochemistry of paraffin-embedded human gastric cancer using MAD2L2 antibody (144-09861) at dilution of 1:100 (40x lens).
Immunohistochemistry - MAD2L2 antibody (144-09861)

Expiration:

12 months from the date of shipment when stored properly.

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