3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-MAGEA1 Antibody
Catalog #: 144-05470
MAGEA1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P43355 |
| Gene Id | 4100 |
| Gene Symbols | MAGEA1 |
| Protein Name / Synonyms | MAGEA1, CT1.1, MAGE1, MAGE family member A1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
34kDa
Observed MW
45kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human MAGEA1 (NP_004979.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of 22Rv1 cells, using MAGEA1 antibody (144-05470) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human breast cancer using MAGEA1 antibody (144-05470) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using MAGEA1 antibody (144-05470) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach cancer using MAGEA1 antibody (144-05470) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using MAGEA1 antibody (144-05470). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.