Anti-MAGED1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9Y5V3
Gene Id	9500
Gene Symbols	MAGED1
Protein Name / Synonyms	MAGED1, DLXIN-1, NRAGE, MAGE family member D1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene is a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although the protein encoded by this gene shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. This gene has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway. Three transcript variants encoding two different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

86kDa/91kDa

Observed MW

86kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 635-834 of human MAGED1 (NP_001005333.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using MAGED1 antibody (144-01092) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - MAGED1 antibody (144-01092)

Immunohistochemistry of paraffin-embedded human liver cancer using MAGED1 antibody (144-01092) at dilution of 1:100 (40x lens).
Immunohistochemistry - MAGED1 antibody (144-01092)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products