Anti-MAP1LC3A Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9H492
Gene Id	84557
Gene Symbols	MAP1LC3A
Protein Name / Synonyms	MAP1LC3A, ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3, microtubule-associated proteins 1A/1B light chain 3A
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.01% thimerosal, 50% glycerol, pH 7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

14kDa

Observed MW

16kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-121 of human MAP1LC3A (NP_115903.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using MAP1LC3A antibody (144-05618) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.
Western blot - MAP1LC3A Polyclonal Antibody (144-05618)

Immunofluorescence analysis of HeLa cells using MAP1LC3A antibody (144-05618).
Immunofluorescence - MAP1LC3A Polyclonal Antibody (144-05618)

Expiration:

12 months from the date of shipment when stored properly.

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