3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-MAP1LC3B Antibody
Catalog #: 144-11282
MAP1LC3B Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9GZQ8 |
| Gene Id | 81631 |
| Gene Symbols | MAP1LC3B |
| Protein Name / Synonyms | MAP1LC3B, ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a, microtubule associated protein 1 light chain 3 beta |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
14kDa
Observed MW
14kDa, 16kDa
Immunogen
A synthetic peptide of human MAP1LC3B
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using MAP1LC3B antibody (144-11282) at 1:1000 dilution. Hela cells were treated by Chloroquine (50 ?M) for 20 hours. NIH/3T3 cells were treated by Chloroquine (50 ?M) for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot analysis of extracts from normal (control) and MAP1LC3B knockout (KO) 293T cells, using MAP1LC3B antibody (144-11282) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using MAP1LC3B antibody (144-11282) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using MAP1LC3B antibody (144-11282) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using MAP1LC3B antibody (144-11282) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using MAP1LC3B antibody (144-11282) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using MAP1LC3B antibody (144-11282) at dilution of 1:100. Hela cells were treated by Chloroquine (50 ?M) for 20 hours(left). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using MAP1LC3B antibody (144-11282) at dilution of 1:100. NIH/3T3 cells were treated by Chloroquine (50 ?M) for 20 hours(left). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.