Anti-MAP2K1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q02750
Gene Id	5604
Gene Symbols	MAP2K1
Protein Name / Synonyms	MAP2K1, CFC3, MAPKK1, MEK1, MKK1, PRKMK1, mitogen-activated protein kinase kinase 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Cardiovascular Disease, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

Dilutions

WB 1:1000 - 1:3000

Format

Calculated MW

40kDa/43kDa

Observed MW

45kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP2K1 (NP_002746.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using MAP2K1 antibody (144-12687) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - [KO Validated] MAP2K1 Polyclonal Antibody (144-12687)

Western blot analysis of extracts from normal (control) and MAP2K1 knockout (KO) HeLa cells, using MAP2K1 antibody (144-12687) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - [KO Validated] MAP2K1 Polyclonal Antibody (144-12687)

Expiration:

12 months from the date of shipment when stored properly.

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