MAP2K5 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q13163 |
| Gene Id | 5607 |
| Gene Symbols | MAP2K5 |
| Protein Name / Synonyms | MAP2K5, HsT17454, MAPKK5, MEK5, PRKMK5, mitogen-activated protein kinase kinase 5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been described.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
46kDa/48kDa/49kDa/50kDa
Observed MW
50kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human MAP2K5 (NP_660143.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using MAP2K5 antibody (144-06953) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.
Immunohistochemistry of paraffin-embedded rat spinal cord using MAP2K5 Antibody (144-06953) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using MAP2K5 Antibody (144-06953) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using MAP2K5 Antibody (144-06953) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using MAP2K5 Antibody (144-06953) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using MAP2K5 antibody (144-06953). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.