Anti-MAP2K7 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O14733
Gene Id	5609
Gene Symbols	MAP2K7
Protein Name / Synonyms	MAP2K7, JNKK2, MAPKK7, MEK, MEK 7, MKK7, PRKMK7, SAPKK-4, SAPKK4, MEK7, mitogen-activated protein kinase kinase 7
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

47kDa/48kDa/49kDa/51kDa

Observed MW

52kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human MAP2K7 (NP_660186.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using MAP2K7 antibody (144-02186) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - MAP2K7 Polyclonal Antibody (144-02186)

Immunofluorescence analysis of U2OS cells using MAP2K7 antibody (144-02186). Blue: DAPI for nuclear staining.
Immunofluorescence - MAP2K7 Polyclonal Antibody (144-02186)

Expiration:

12 months from the date of shipment when stored properly.

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