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Anti-MAP3K3 Antibody
Catalog #: 144-64058
MAP3K3 pAb
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q99759 |
| Gene Id | 4215 |
| Gene Symbols | MAP3K3 |
| Protein Name / Synonyms | MAP3K3, MAPKKK3, MEKK3, mitogen-activated protein kinase kinase kinase 3 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Dilutions
WB 1:500 - 1:2000; IHC 1:100 - 1:200
Format
Calculated MW
70kDa/74kDa
Observed MW
71kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human MAP3K3 (NP_976226.1).
Modification
Unmodified
Storage
Store at -20°. Avoid freeze / thaw cycles.
Images
Expiration:
12 months from the date of shipment when stored properly.