Anti-MAP4K3 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q8IVH8
Gene Id	8491
Gene Symbols	MAP4K3
Protein Name / Synonyms	MAP4K3, GLK, MAPKKKK3, MEKKK 3, MEKKK3, RAB8IPL1, mitogen-activated protein kinase kinase kinase kinase 3
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the mitogen-activated protein kinase kinase kinase kinase family. The encoded protein activates key effectors in cell signalling, among them c-Jun. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

98kDa/100kDa/101kDa

Observed MW

110kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 755-894 of human MAP4K3 (NP_003609.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using MAP4K3 antibody (144-07351) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - MAP4K3 antibody (144-07351)

Immunohistochemistry of paraffin-embedded human liver injury using MAP4K3 antibody (144-07351) at dilution of 1:100 (40x lens).
Immunohistochemistry - MAP4K3 antibody (144-07351)

Expiration:

12 months from the date of shipment when stored properly.

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