[KO Validated] MAPK6 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q16659 |
| Gene Id | 5597 |
| Gene Symbols | MAPK6 |
| Protein Name / Synonyms | MAPK6, ERK3, HsT17250, PRKM6, p97MAPK, mitogen-activated protein kinase 6 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | MAPK Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.
Dilutions
WB 1:200 - 1:2000
Format
Calculated MW
82kDa
Observed MW
120kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 372-721 of human MAPK6 (NP_002739.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using MAPK6 antibody (144-60988) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.![Western blot - [KO Validated] MAPK6 Polyclonal Antibody (144-60988)](https://cdn.sanity.io/images/g51potcz/production/fd95be55018dded1f89ed59a6d0a90a8819f21b0-303x350.jpg)
Western blot analysis of extracts from normal (control) and MAPK6 knockout (KO) HeLa cells, using MAPK6 antibody (144-60988) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.![Western blot - [KO Validated] MAPK6 Polyclonal Antibody (144-60988)](https://www.raybiotech.com/files/images/Antibodies/144/144-60988/2.jpg)
Expiration:
12 months from the date of shipment when stored properly.