Anti-MAPK9 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P45984
Gene Id	5601
Gene Symbols	MAPK9
Protein Name / Synonyms	MAPK9, JNK-55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, PRKM9, SAPK, SAPK1a, p54a, p54aSAPK, mitogen-activated protein kinase 9
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Inflammation, MAPK Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

27kDa/44kDa/48kDa

Observed MW

54kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-424 of human MAPK9 (NP_002743.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using MAPK9 antibody (144-01251) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - [KO Validated] MAPK9 Polyclonal Antibody (144-01251)

Western blot analysis of extracts from normal (control) and MAPK9 knockout (KO) 293T cells, using MAPK9 antibody (144-01251) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - [KO Validated] MAPK9 Polyclonal Antibody (144-01251)

Immunohistochemistry of paraffin-embedded human breast cancer using MAPK9 antibody (144-01251) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] MAPK9 Polyclonal Antibody (144-01251)

Immunohistochemistry of paraffin-embedded mouse brain using MAPK9 antibody (144-01251) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] MAPK9 Polyclonal Antibody (144-01251)

Immunofluorescence analysis of U2OS cells using MAPK9 antibody (144-01251). Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] MAPK9 Polyclonal Antibody (144-01251)

Expiration:

12 months from the date of shipment when stored properly.

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