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Anti-MAPKBP1 Antibody
Catalog #: 144-02626
MAPKBP1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O60336 |
| Gene Id | 23005 |
| Gene Symbols | MAPKBP1 |
| Protein Name / Synonyms | MAPKBP1, JNKBP-1, JNKBP1, NPHP20, mitogen-activated protein kinase-binding protein 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Cardiovascular Disease, MAPK Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a scaffold protein that regulates the JNK (c-Jun N-terminal kinase) and NOD2 (nucleotide-binding oligomerization domain-containing protein 2) signaling pathways. The encoded protein interacts with another related JNK pathway scaffold protein, WDR62, via a conserved dimerization domain, and enhances JNK signaling. This protein may play a role in bacterial immunity by binding to the NOD2 receptor and negatively regulating downstream antibacterial and pro-inflammatory signaling. Mutations in this gene that impair cellular localization of the encoded protein cause a form of nephronophthisis, an autosomal-recessive kidney disorder, in human patients.
Dilutions
WB 1:200 - 1:500
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
23kDa/109kDa/134kDa/149kDa/163kDa
Observed MW
165kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAPKBP1 (NP_001122080.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using MAPKBP1 antibody (144-02626) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded mouse stomach using MAPKBP1 antibody (144-02626) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using MAPKBP1 antibody (144-02626) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.