RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

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ISO 13485:2016 cGMP

Anti-MAPRE3 Antibody

Catalog #: 144-09591

MAPRE3 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesMouse
Host SpeciesRabbit
Accession Number
Q9UPY8
Gene Id
22924
Gene Symbols
MAPRE3
Protein Name / Synonyms
MAPRE3, EB3, EBF3, EBF3-S, RP3, microtubule-associated protein RP/EB family member 3
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene is a member of the RP/EB family of genes. The protein localizes to the cytoplasmic microtubule network and binds APCL, a homolog of the adenomatous polyposis coli tumor suppressor gene.

Dilutions

WB 1:1000 - 1:3000
IHC 1:50 - 1:200

Format

Calculated MW
30kDa/31kDa
Observed MW
35kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-281 of human MAPRE3 (NP_036458.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using MAPRE3 antibody (144-09591) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - MAPRE3 antibody (144-09591)

Immunohistochemistry of paraffin-embedded mouse bronchus using MAPRE3 antibody (144-09591) at dilution of 1:100 (40x lens).
Immunohistochemistry - MAPRE3 antibody (144-09591)

Expiration:
12 months from the date of shipment when stored properly.