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Anti-MARCKS Antibody
Catalog #: 144-00936
MARCKS Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P29966 |
| Gene Id | 4082 |
| Gene Symbols | MARCKS |
| Protein Name / Synonyms | MARCKS, 80K-L, MACS, PKCSL, PRKCSL, myristoylated alanine-rich C-kinase substrate |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
31kDa
Observed MW
80kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MARCKS (NP_002347.5).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of rat brain, using MARCKS antibody (144-00936) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Expiration:
12 months from the date of shipment when stored properly.