Anti-MBD2 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9UBB5
Gene Id	8932
Gene Symbols	MBD2
Protein Name / Synonyms	MBD2 (Center)
Clonality	Polyclonal
Preparation	This MBD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-279 amino acids from the Central region of human MBD2.
Concentration (lot specific)	0.260 mg/ml
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.

Specificity

Predicted Reactivity

Mouse

Other Names

MBD2; Methyl-CpG-binding domain protein 2; Demethylase; Methyl-CpG-binding protein MBD2

NCBI Accession #

NP_003918.1;NP_056647.1

Other Accession #

Q9Z2E1; NP_056647.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

43255

Recommended Dilutions

IF: 1:10-50; WB: 1:1000

Fluorescent confocal image of Hela cell stained with MBD2 Antibody (Center)(Cat# 102-14469).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MBD2 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).MBD2 immunoreactivity is localized to Nucleus significantly.

MBD2 Antibody (Center) (Cat# 102-14469) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the MBD2 antibody detected the MBD2 protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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