Anti-MBD3 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O95983
Gene Id	53615
Gene Symbols	MBD3
Protein Name / Synonyms	MBD3 (N-term)
Clonality	Polyclonal
Preparation	This MBD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MBD3.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex.

Specificity

Predicted Reactivity

Mouse

Other Names

MBD3; Methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3

NCBI Accession #

NP_001268382.1;NP_001268383.1

Other Accession #

Q9Z2D8; NP_003917.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

32844

Recommended Dilutions

WB: 1:1000

MBD3 Antibody (N-term) (Cat# 102-12141) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the MBD3 antibody detected the MBD3 protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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