Anti-MBP (Ascites) Antibody

Product Description

Specifications

Size	0.1 mL
Estimated Lead Time	1-2 weeks
Species	Human
Accession Number	P02686
Gene Id	4155
Gene Symbols	MBP
Protein Name / Synonyms	MBP (Ascites)
Clonality	Monoclonal
Preparation	Purified His-tagged MBP protein(Fragment) was used to produced this monoclonal antibody.
Recommended Applications	Western Blotting, IF
Research Area	Neuroscience
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called 'Golli-MBP') that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq].

Specificity

Other Names

MBP; Myelin basic protein; Myelin A1 protein; Myelin membrane encephalitogenic protein

NCBI Accession #

NP_001020252.1;NP_001020261.1;NP_001020263.1;NP_001020271.1;NP_001020272.1;NP_002376.1

Other Accession #

NP_001020261.1; NP_001020252.1

Format

Type

Mouse Monoclonal Antibody (Mab)

Calculated Molecular Weight (Da)

33117

Recommended Dilutions

WB: 1:5000-16000; IF: 1:10-50

	MBP Antibody (Ascites)(Cat# 102-10235) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the MBP antibody detected the MBP protein (arrow).
	Confocal immunofluorescent analysis of MBP Antibody (Ascites)(Cat# 102-10235) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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