3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-MCF2 Antibody
Catalog #: 144-12839
MCF2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P10911 |
| Gene Id | 4168 |
| Gene Symbols | MCF2 |
| Protein Name / Synonyms | MCF2, ARHGEF21, DBL, proto-oncogene DBL |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The oncogenic protein encoded by this gene is a guanine nucleotide exchange factor (GEF) that exerts control over some members of the Rho family of small GTPases. Several transcript variants encoding different isoforms have been found for this gene. These isoforms exhibit different expression patterns and varying levels of GEF activity.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
95kDa/100kDa/107kDa/109kDa/114kDa/116kDa
Observed MW
108kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 872-1001 of human MCF2 (NP_001165347.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of HT-29 cells, using MCF2 antibody (144-12839) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Expiration:
12 months from the date of shipment when stored properly.