RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

www.RayBiotech.com | info@raybiotech.com

770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-MELK Antibody

Catalog #: 144-10794

MELK Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q14680
Gene Id
9833
Gene Symbols
MELK
Protein Name / Synonyms
MELK, HPK38, maternal embryonic leucine zipper kinase
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Research AreaCancer
Shipping TypeBlue ice
Storage-20°C

Description

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
52kDa/59kDa/66kDa/69kDa/70kDa/71kDa/74kDa
Observed MW
75kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 412-651 of human MELK (NP_055606.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using MELK antibody (144-10794) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - MELK Polyclonal Antibody (144-10794)

Expiration:
12 months from the date of shipment when stored properly.