Anti-MERTK Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q12866
Gene Id	10461
Gene Symbols	MERTK
Protein Name / Synonyms	MERTK, MER, RP38, Tyro12, c-Eyk, c-mer, tyrosine-protein kinase Mer
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP).

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

110kDa

Observed MW

160kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 840-999 of human MERTK (NP_006334.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using MERTK antibody (144-05443) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Western blot - MERTK antibody (144-05443)

Immunofluorescence analysis of U2OS cells using MERTK antibody (144-05443) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - MERTK antibody (144-05443)

Expiration:

12 months from the date of shipment when stored properly.

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