Anti-MGAT3 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q09327
Gene Id	4248
Gene Symbols	MGAT3
Protein Name / Synonyms	MGAT3, GNT-III, GNT3, beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. The enzyme encoded by this gene transfers a GlcNAc residue to the beta-linked mannose of the trimannosyl core of N-linked oligosaccharides and produces a bisecting GlcNAc. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW

61kDa

Observed MW

61kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 364-533 of human MGAT3 (NP_002400.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using MGAT3 antibody (144-08134) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Western blot - MGAT3 antibody (144-08134)

Immunohistochemistry of paraffin-embedded rat heart using MGAT3 antibody (144-08134) at dilution of 1:100 (40x lens).
Immunohistochemistry - MGAT3 antibody (144-08134)

Expiration:

12 months from the date of shipment when stored properly.

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