Anti-MGMT Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P16455
Gene Id	4255
Gene Symbols	MGMT
Protein Name / Synonyms	MGMT, O-6-methylguanine-DNA methyltransferase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

21kDa

Observed MW

22kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human MGMT (XP_005252739.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of HepG2 cells, using MGMT antibody (144-00693) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - MGMT Polyclonal Antibody (144-00693)

Immunofluorescence analysis of U2OS cells using MGMT antibody (144-00693). Blue: DAPI for nuclear staining.
Immunofluorescence - MGMT Polyclonal Antibody (144-00693)

Expiration:

12 months from the date of shipment when stored properly.

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