Anti-MGMT (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P16455
Gene Id	4255
Gene Symbols	MGMT
Protein Name / Synonyms	MGMT (N-term)
Clonality	Polyclonal
Preparation	This MGMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MGMT.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

MGMT is involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Specificity

Other Names

MGMT; Methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

21646

Recommended Dilutions

WB: 1:1000; IF: 1:10-50

	MGMT Antibody (N-term) (Cat# 102-12191) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the MGMT antibody detected the MGMT protein (arrow).
	Confocal immunofluorescent analysis of MGMT Antibody (N-term)(Cat# 102-12191) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products