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Anti-MIP Antibody
Catalog #: 144-02886
MIP Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P30301 |
| Gene Id | 4284 |
| Gene Symbols | MIP |
| Protein Name / Synonyms | MIP, AQP0, CTRCT15, LIM1, MIP26, MP26, lens fiber major intrinsic protein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Major intrinsic protein is a member of the water-transporting aquaporins as well as the original member of the MIP family of channel proteins. The function of the fiber cell membrane protein encoded by this gene is undetermined, yet this protein is speculated to play a role in intracellular communication. The MIP protein is expressed in the ocular lens and is required for correct lens function. This gene has been mapped among aquaporins AQP2, AQP5, and AQP6, in a potential gene cluster at 12q13.
Dilutions
WB 1:500 - 1:1000
IHC 1:100 - 1:200
IHC 1:100 - 1:200
Format
Calculated MW
28kDa
Observed MW
28kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human MIP (NP_036196.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using MIP antibody (144-02886) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse brain using MIP antibody (144-02886) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using MIP antibody (144-02886) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using MIP antibody (144-02886) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.