Anti-MITF (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O75030
Gene Id	4286
Gene Symbols	MITF
Protein Name / Synonyms	MITF (N-term)
Clonality	Polyclonal
Preparation	This MITF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-28 amino acids from the N-terminal region of human MITF.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IF, FC
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.

Specificity

Predicted Reactivity

Mouse

Other Names

MITF; BHLHE32; Microphthalmia-associated transcription factor; Class E basic helix-loop-helix protein 32

NCBI Accession #

NP_000239.1;NP_001171896.1;NP_006713.1;NP_937801.1;NP_937802.1;NP_937820.1;NP_937821.2

Other Accession #

Q08874; NP_937802.1; NP_001171896.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

58795

Recommended Dilutions

WB: 1:1000; IF: 1:10-50; FC: 1:10-50

	MITF Antibody (N-term) (Cat# 102-11148) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MITF antibody detected the MITF protein (arrow).
	Confocal immunofluorescent analysis of MITF Antibody (N-term)(Cat# 102-11148) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
	MITF Antibody (N-term) (Cat# 102-11148) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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