Anti-MKI67 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P46013
Gene Id	4288
Gene Symbols	MKI67
Protein Name / Synonyms	MKI67, KIA, MIB-, MIB-1, PPP1R105, marker of proliferation Ki-67
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100

Format

Calculated MW

319kDa/358kDa

Observed MW

360kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1200-1300 of human MKI67 (NP_001139438.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of HeLa cells, using MKI67 antibody (144-02094) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - MKI67 Polyclonal Antibody (144-02094)

Immunofluorescence analysis of HeLa cells using MKI67 antibody (144-02094) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - MKI67 Polyclonal Antibody (144-02094)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products