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Anti-MLF1 Antibody
Catalog #: 144-08012
MLF1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P58340 |
| Gene Id | 4291 |
| Gene Symbols | MLF1 |
| Protein Name / Synonyms | MLF1, myeloid leukemia factor 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes an oncoprotein which is thought to play a role in the phenotypic determination of hemopoetic cells. Translocations between this gene and nucleophosmin have been associated with myelodysplastic syndrome and acute myeloid leukemia. Multiple transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:100
IF 1:50 - 1:100
Format
Calculated MW
23kDa/27kDa/29kDa/30kDa/33kDa
Observed MW
35kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-268 of human MLF1 (NP_071888.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using MLF1 antibody (144-08012) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunofluorescence analysis of A549 cells using MLF1 antibody (144-08012).
Expiration:
12 months from the date of shipment when stored properly.