Anti-MNAT1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P51948
Gene Id	4331
Gene Symbols	MNAT1
Protein Name / Synonyms	MNAT1, CAP35, MAT1, RNF66, TFB3, CDK-activating kinase assembly factor MAT1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IP
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene, along with cyclin H and CDK7, forms the CDK-activating kinase (CAK) enzymatic complex. This complex activates several cyclin-associated kinases and can also associate with TFIIH to activate transcription by RNA polymerase II. Two transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IP 1:50 - 1:200

Format

Calculated MW

31kDa/35kDa

Observed MW

36kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human MNAT1 (NP_002422.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using MNAT1 antibody (144-01918) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - MNAT1 Polyclonal Antibody (144-01918)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug MNAT1 antibody (144-01918). Western blot was performed from the immunoprecipitate using MNAT1 antibody (144-01918) at a dilition of 1:1000.
Immunoprecipitation - MNAT1 Polyclonal Antibody (144-01918)

Expiration:

12 months from the date of shipment when stored properly.

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