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Anti-MonoMethyl-Histone H3-R17 Antibody
Catalog #: 144-03151
MonoMethyl-Histone H3-R17 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P68431 |
| Gene Id | 8356 |
| Gene Symbols | H3R17ME1 |
| Protein Name / Synonyms | HIST1H3J, H3/j, H3FJ, histone H3.1 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP, ChIP, ChIPseq |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
CHIPseq 1:20 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
CHIPseq 1:20 - 1:100
Format
Calculated MW
15kDa
Observed MW
17kDa
Immunogen
A synthetic mono-methylated peptide corresponding to residues surroundingArg17 of human histone H3
Modification
MonoMethyl R17
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-R17 antibody (144-03151).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using MonoMethyl-Histone H3-R17 antibody (144-03151).
Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H3-R17 antibody (144-03151). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.